The Biodiversity Survey of the Cape (BioSCape) is an international collaboration between South Africa and the United States to study biodiversity in South Africa’s Greater Cape Floristic Region (GCFR). The GCFR was selected due to two exceptional hotspots of both terrestrial and aquatic biodiversity. The GCFR is listed among the World’s 200 Significant Ecoregions. The BioSCape is an integrated field and airborne campaign occurring in 2023. The campaign will collect UV/visible to short wavelength infrared (UVSWIR) and thermal imaging spectroscopy and laser altimetry LiDAR data over terrestrial and aquatic targets using four airborne instruments: Airborne Visible InfraRed Imaging Spectrometer - Next Generation (AVIRIS-NG), Portable Remote Imaging SpectroMeter (PRISM), Land, Vegetation, and Ice Sensor (LVIS), and Hyperspectral Thermal Emission Spectrometer (HyTES). The anticipated airborne data set is unique in its size and scope and unprecedented in its instrument combination and level of detail. These airborne data will be accompanied by a range of biodiversity-related field observations. BioSCape’s primary objective is to understand the structure, function, and composition of the region’s ecosystems, and to learn about how and why they are changing in time and space.